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Juvenile hormone production, juvenile hormone esterase, and juvenile hormone acid methyltransferase in corpora allata ofManduca sexta

✍ Scribed by Sparagana, Steven P. ;Bhaskaran, Govindan ;Dahm, Karl H. ;Riddle, Vicki

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 533 KB
Volume: 230
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402300216

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✦ Synopsis

Juvenile hormone (JH) secretion by corpora allata (CA) of last instar larvae of Manduca sexta was studied by measuring the rate of JH synthesis in vitro with a radiochemical assay and by analyzing JH esterase and JH acid methyltransferase (JHAMT) activities in CA. CA of 0-day (0-12 hr after ecdysis), 4-day (early wandering larvae), and 7-day (prepupae) animals were used in this study.

CA of 0-day larvae showed high rates of JH synthesis, whereas CA of both 4and 7-day animals did not produce detectable amounts of radiolabeled JH. JH esterase activity was absent in 0-day CA, but high activity was present in both 4-and 7-day CA. However, incubation of older CA in medium containing

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