This book is intended as a comprehensive and authoritative collection of methods employed in electron microscopic immunocytochemistry and proves that this is a technique of tremendous power, combining the rcsolution of the electron microscope with the specificity and sensitivity of immunocytochcmistry. The 10 chapters, written by 16 prominent researchers in this field, might be divided into two main parts. The first 5 chapters cover the principles behind the technique and give detailed information on the procedures involved in post-and pre-embedding staining and in the preparation of ultrathin cryosections. The concluding 5 chapters demonstrate the application of electon microscopic immunocytochemistry to the study of endocrine tumours, renal diseases, neuropathology, dermatology, and microbiology. Each chapter is supplemented with a methodical appendix and a list of references. The book is completed by a sufficient index, an appendix, describing the preparation of general solutions used in electron microscopic immunocytochemistry, and brings a list of suppliers of reagcnts and equipment used in electron microscopic immunocytochemistry.
The appearance of this volume is very timely. This book makes clcctron microscopic immunocytochemistry more accessible by providing a thorough introduction to thc technique and its applications in biomedical research and in diagnostic histopathology. The book will be invaluable to clinical and research pathologists, electron microscopists, histochemists, and scientists studying cellular functions in almost any tissue.
ICOR B. BUCHWALOW (Jena/Moscow)