✦ LIBER ✦
J-Modulated TROSY Experiment Extends the Limits of Homonuclear Coupling Measurements for Larger Proteins
✍ Scribed by Katalin E. Kövér; Gyula Batta
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 90 KB
- Volume
- 151
- Category
- Article
- ISSN
- 1090-7807
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✦ Synopsis
This paper describes the use of a TROSY experimental scheme and its variant extended with a scaled J-modulation spin-echo sequence for accurate and sensitive measurement of homonuclear 3J(H(N)H(alpha)) coupling constants in larger proteins with uniform 15N labeling. Exclusive selection of the most slowly relaxing component of a 15N-1H multiplet by the TROSY approach leads to substantial improvement in resolution; this is a prerequisite for accurate measurement of couplings from the 1H multiplets directly along the 1H frequency dimension or from the J-scaled doublets along the 15N frequency dimension.