Isozyme variation between diploid and tetraploid cytotypes ofGlycine tabacina(Labill.) Benth.
✍ Scribed by D. I. Menancio; T. Hymowitz
- Book ID
- 104620074
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 545 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0014-2336
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✦ Synopsis
Glycine tabacina (Labill .) Benth . is a wild perennial species related to the cultivated soybean, G . max (L .) Merr . It is composed of diploid (2n = 40) and tetraploid (2n = 80) cytotypes . Currently, to differentiate the cytotypes, plants are grown out in the greenhouse and chromosome counts made on pollen mother cells. It is a laborious and time consuming process . The objective of this study was to determine whether electrophoretic techniques could be utilized to separate the cytotypes . Electrophoretic examination of seven isozyme systems from seed of 67 G. tabacina accessions revealed banding patterns that could be used to differentiate between diploid and tetraploid cytotypes in the species . Among the tetraploid accessions, the number of bands observed were always greater than the diploids . Some tetraploid banding patterns consisted of bands similar to the diploid tabacina and/or additional bands previously identified in other Glycine species. The patterns of isozyme multiplicity and variation in the tetraploid tabacinas suggests more than one mode of origin for the tetraploids .