Isozyme differentiation ofAzollaLam

✍ Scribed by William J. Zimmerman; Thomas A. Lumpkin; Iwao Watanabe

• Book ID: 104620084
• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 576 KB
• Volume: 42
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00042628

✦ Synopsis

Enzyme electrophoresis was used to differentiate sections within the genus Azolla Lam . and demonstrate the value of this method in fingerprinting taxa . Polyacrylamide gel methodology has been developed to identify suspected species of this genus . Preliminary examination of selected isolates indicated that allozyme diversity was particularly evident in section Azolla, where specific classification by morphological means is difficult . Leaf tissue was preferable to root tissue for this purpose because of the ease of tissue preparation and the superior resolution and staining intensity of enzymes . Contribution of enzyme bands by the cyanobiont to zymograms was not significant .