Isotopic discrimination between D-[1-13C]fructose and D-[2-13C]fructose in rat liver cells
β Scribed by Willy J. Malaisse; Rudolph Willem
- Book ID
- 116308283
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 181 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0300-9084
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## Abstract Tumoral pancreatic islet cells of the RIN5mF line were incubated for 120 min in media prepared in ^2^H~2~O and containing Dβ[1β^13^C]glucose, and Dβ[2β^13^C]glucose, and Dβ[6β^13^C]glucose. The generation of C~2~β and C~3~β deuterated lactic acid was assessed by ^13^C NMR. The interpret
Three isotope-edited n.m.r. methods have been applied to selectively 13C-substituted monosaccharides and nucleosides to simplify their spectra and/or measure 1H-1H, 13C-1H, or 13H-13C spin-couplings detected via the labeled site. 1D INADEQUATE spectra allowed the selective detection of the natural-a