Isomer and congener identification of chlorinated pyrenes by narrow-bore liquid chromatography—Fourier transform infrared spectrometry

✍ Scribed by G.W. Somsen; L.P.P. van Stee; C. Gooijer; U.A.Th. Brinkman; N.H. Velthrost; T. Visser

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 688 KB
• Volume: 290
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)80113-4

✦ Synopsis

Liquid chromatography (LC) was coupled semi on-line to Fourier transform infrared spectrometry (IT-IR) using a spray-jet assembly interface to eliminate the LC eluent prior to IR detection. The usefulness of the LC-IT-IR system in the identification of closely related compounds in complex mixtures is demonstrated by the analysis of a chlorinated pyrene sample which contains a number of chloropyrene isomers and congeners. Characteristic FT-IR transmission spectra of all constituents could be recorded. Since most of these compounds have not been analysed by IR before, spectral assignment was mainly based on the empirical Hansen-Berg rules for substituted pyrenes. The identification limits for the chloropyrenes typically were 5-10 ng injected.