Isolierung und Charakterisierung von DNA-Abbaumutanten bei Proteus mirabilis: Funktion der Endonuclease I im postmortalen DNA-Abbau
✍ Scribed by Dr. G. Steinborn; J. Hofemeister; H. Böhme
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 591 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
DNase deficient mutants of Proteus mirabilis selected for reduced toluene induced DNA degradation were isolated. Their defect in DNA degradation was shown not only after treatment by toluene but also in crude extracts after cell disintegration by ultrasonic and in untreated starved cultures. The degradation mutants behave just as the wild type with respect to their in vivo functions proofed. The results indicate that the affected DNase does not have an essential function in vivo but acts in postmortem DNA degradation. Probably, the DNase in question concerns the endonuclease I of P. mirabilis described by GOEBEL and HELINSKI (1971 a, b).