✦ LIBER ✦

Isolation of γ phage DNA by hydroxylapatite chromatography

✍ Scribed by Ivan Ivanov; Lilly Gigova

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 590 KB
Volume: 146
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90556-1

No coin nor oath required. For personal study only.

✦ Synopsis

A simple and rapid (1 day) method for preparation of lambda phage DNA was proposed. The method included two main steps: (a) growth and lysis of bacteria containing lambda phage and (b) purification of lambda phage DNA by hydroxylapatite chromatography. The phage DNA prepared by this method was intact and free of RNA, proteins, and bacterial DNA.

📜 SIMILAR VOLUMES

Isolation of maltopentaose from corn syr

Isolation of maltopentaose from corn syrup by chromatography on granulated hydroxylapatite

✍ W.Terry Jenkins 📂 Article 📅 1979 🏛 Elsevier Science 🌐 English ⚖ 333 KB

Continuous isolation of plasmid DNA by a

Continuous isolation of plasmid DNA by annular chromatography

✍ R. Giovannini; R. Freitag 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 723 KB

A Preparation of λ Phage DNA Based on Af

A Preparation of λ Phage DNA Based on Affinity Chromatography

✍ E. Coto; T.E. Hugli; R.D. Ye; R.G. Discipio 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 311 KB

Isolation of Single-Stranded DNA Using D

Isolation of Single-Stranded DNA Using Denaturing DNA Chromatography

✍ Mark Dickman; David P. Hornby 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 58 KB

The versatility of the modified micro-Bradford CaPE assay was supported by the results with the strongly glycosylated glycophorin, which did not deviate excessively from the other protein preparations either in the rate of color development or in the sensitivity, whereas it showed very slow color de

Selective isolation of mercurated DNA by

Selective isolation of mercurated DNA by affinity chromatography on thiol matrices

✍ Gaspar Banfalvi; Sudha Bhattacharya; Nilima Sarkar 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 740 KB

A method for isolating picomole quantities of nascent mercurated DNA from a mixture of cellular nucleic acids using affinity chromatography on thiol-agarose is described. Analysis of mercurated DNA (HgDNA) isolated in the presence of in vivo-labeled cellular RNA or in vitro-synthesized RNA showed a

Isolation of high-rate DNA synthetic cel

Isolation of high-rate DNA synthetic cells by Con A chromatography

✍ Mark A. Wallenbrock; Jerald J. Killion 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 English ⚖ 604 KB

## Abstract The separation of L1210 cells with columns of Con A‐derivatized nylon was investigated. Most of the cells bound to the column irreversibly. The binding was lectin‐specific. Cells were pulse labeled with ^3^H‐thymidine and applied to Con A columns. Those cells not binding the columns wer