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Isolation of Vulgin, a New Antifungal Polypeptide with Mitogenic Activity from the Pinto Bean

✍ Scribed by X. Y. Ye; Dr T. B. Ng

Publisher: John Wiley and Sons
Year: 2003
Tongue: English
Weight: 155 KB
Volume: 9
Category: Article
ISSN: 1075-2617
DOI: 10.1002/psc.436

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✦ Synopsis

Abstract

An antifungal polypeptide bearing an N‐terminal sequence with some homology to chitinases was purified from an extract of pinto beans. The polypeptide, designated vulgin, exerted antifungal activity toward Mycosphaerella arachidicola, Coprinus comatus, Fusarium oxysporum and Botrytis cinerea. Vulgin inhibited translation in a rabbit reticulocyte lysate system with an IC~50~ of 4.3 µM and HIV‐1 reverse transcriptase activity with an IC~50~ of 58 µM. Vulgin stimulated in vitro incorporation of methyl [^3^H] thymidine into mouse splenocytes. Copyright © 2003 European Peptide Society and John Wiley & Sons, Ltd.

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