Isolation of two different polysaccharides from halophilicZoogloeasp.
β Scribed by Kyung-Ja Kwon; Kee-Jai Park; Jong-Deog Kim; Jai-Yul Kong; In-Soo Kong
- Publisher
- Springer Netherlands
- Year
- 1994
- Tongue
- English
- Weight
- 379 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0141-5492
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β¦ Synopsis
An extracellular polysaccharide producing bacterium Zbogloea sp. was isolated from marine environments.
This strain could produce two different polysaccharides. One ( water-soluble polysaccharide : WSP 1 was from cell-free liquid medium, the other ( cell-bound polysaccharide :
CBP 1 was obtained from cell surface. Both polysaccharides contained glucose, galactose and mannose as sugar components, but their molar ratios were different ( WSP : 2~2~3, CBP : 1:2:2 1 and half of the sugar components existed as uranic acid form. Both polysaccharide productions started at the early stage of the logarithmic growth phase. The amount of WSP and CBP was influenced by culture conditions such as additional carbon and nitrogen sources. Isolated Z%og.!oea sp. showed a high product yield without the increase of cell mass.
π SIMILAR VOLUMES
Cell-wall material was prepared from unripe tomato fruit, and a pectic polysaccharide extracted with cyclohexanediaminetetraacetic acid. The structure of the purified pectic polysaccharide was examined by sugar and methylation analysis, and was typical of a rhamnogalacturonan from the primary cell w