Isolation of two antigens from the culture filtrates ofMycobacterium tuberculosisand their applications in the laboratory diagnosis of the tuberculous meningitis

✍ Scribed by V. V. Krishnan; A. Mathai

• Publisher: Springer-Verlag
• Year: 1991
• Tongue: English
• Weight: 613 KB
• Volume: 180
• Category: Article
• ISSN: 0300-8584
• DOI: 10.1007/bf00193851

✦ Synopsis

Two antigens were isolated from the culture filtrates of H37Ra Mycobacterium tuberculosis by immunoabsorbent affinity chromatography, M. tuberculosis antigen 5 and immunoabsorbent affinity column-purified antigen (IAP). The potential application of these two mycobacterial antigens in the laboratory diagnosis of tuberculous meningitis was evaluated by indirect enzymelinked immunoabsorbent assay in cerebrospinal fluid specimens. IAP antigen was more sensitive than antigen 5, although antigen 5 was more specific than lAP antigen in detecting tuberculous aetiology. Technical aspects of immunoabsorbent affinity chromatography have been highlighted in this study.