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Isolation of three allergenic fractions of the major allergen from Olea europea pollen and N-terminal amino acid sequence

✍ Scribed by Mayte Villalba; Carlos López-Otín; Elena Martín-Orozco; Rafael I. Monsalve; Pilar Palomino; Carlos Lahoz; Rosalía Rodríguez


Book ID
115762873
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
396 KB
Volume
172
Category
Article
ISSN
0006-291X

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Considering the known N-terminal amino acid sequence of the major apple allergen, a polymerase chain reaction (PCR) primer was selected to amplify cDNA encoding this protein. A single PCR product was obtained, cloned into Escherichia coli and subsequently sequenced. The missing 5'-end of the apple c