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Isolation of the C-terminal fragment of proteins after re-digestion of their esterified tryptic hydrolysates

✍ Scribed by DIBÓ, GÁBOR ;FURKA, ÁRPÁD ;SEBESTYÉN, FERENC ;FEHÉRVÁRI, ÁGOTA


Book ID
115098401
Publisher
Wiley (Blackwell Publishing)
Year
2009
Tongue
English
Weight
452 KB
Volume
27
Category
Article
ISSN
0367-8377

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A novel method for selective isolation o
✍ Takashi Kumazaki; Tatsushi Nakako; Fumio Arisaka; Shin-Ichi Ishii 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 760 KB

A novel method useful for selective isolation of the C-terminal peptide from a tryptic digestion mixture of a protein has been developed by taking advantage of a unique property of anhydrotrypsin, which has a strong specific affinity for the peptides containing arginine or lysine at their C-termini.