Isolation of the anti-inflammatory principles from Achillea millefolium (compositae)

✍ Scribed by Arthur S. Goldberg; Evangelynne C. Mueller; Edward Eigen; Salvatore J. Desalva

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: English
• Weight: 461 KB
• Volume: 58
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600580805

✦ Synopsis

was obtained by summation of radioactivity in each spot from a sample applied to the thin-layer plate.

As may be observed in Table V, from five to seven labeled compounds were separated from urine samples collected at various intervals after dosing. The compound at R f 0.50 corresponded to intact furosemide while the compound at R f 0.41 was 4-chloro-5sulfamyl anthranilic acid. The remaining labeled compounds were not identified. The relative amount of furosemide decreased with the passage of time, while metabolites of furosemide increased. The 24-hr. urine sample contained 40 to 47% of total radioactivity as furosemide and approximately 30 as 4chloro-5-sulfamyl anthranilic acid. The labeled compound at R f 0.24 contained up to 2 0 z of the total radioactivity in the 24-hr. urine sample. The methanol solution of 35S-labeled furosemide applied as a reference to the chromatographic plate contained 2.5 % 4-chloro-5-sulfamyl anthranilic acid as a radiochemical impurity.