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Isolation of Temperature-Sensitive Mutants of L-Cells

โœ Scribed by L. H. Thompson, R. Mankovitz, R. M. Baker, J. E. Till, L. Siminovitch and G. F. Whitmore


Book ID
123649670
Publisher
National Academy of Sciences
Year
1970
Tongue
English
Weight
885 KB
Volume
66
Category
Article
ISSN
0027-8424

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Selective and nonselective isolation of
โœ L. H. Thompson; R. Mankovitz; R. M. Baker; J. A. Wright; J. E. Till; L. Siminovi ๐Ÿ“‚ Article ๐Ÿ“… 1971 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 639 KB

Mutants of mouse L-cells which are temperature-sensitive for growth have been obtained by using both selective and nonselective isolation procedures on populations treated with the mutagen nitrosoguanidine. Selective isolation was carried out by utilizing a five-day treatment with 3H-TdR and ara-C a