Isolation of sindbis virus 26 S RNA by cDNA-cellulose chromatography
โ Scribed by Arturo Leone; Aaron J. Shatkin; Ranieri Cancedda
- Book ID
- 115910291
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 332 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0014-5793
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๐ SIMILAR VOLUMES
A method for the isolation of messenger RNA (mRNA) from polysomes is described. Pofysomes are dissolved in a solution containing 0.5 M NaCl and Na dodecyl sulphate and applied to an oligo(dT)-cellulose column. RNA species containing poly(A) sequences are retained by the column, whereas ribosomal pro
Polyacrylamide and porous-glass supports containing the dihydroxyborylphenyl group can be prepared by a method similar to that used in the synthesis of N-[ N'-(m-dihydroxyborylphenyl)succinamyl]aminoethylcellulose. The reaction of aminoethylpolyacrylamide or amino-substituted glass with N-(m-dihydr