Isolation of simian immunodeficiency virus from african green monkeys and seroepidemiologic survey of the virus in various non-human primates
✍ Scribed by Yoshihiro Ohta; Takao Masuda; Hajime Tsujimoto; Koh-Ichi Ishikawa; Toshiaki Kodama; Shigeru Morikawa; Masuyo Nakai; Shigeo Honjo; Masanori Hayami
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- French
- Weight
- 984 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Sixteen isolates of simian retrovirus closely related to human immunodeficiency virus (HIV) were obtained from healthy African green monkeys (AGM) (Cercopithecus aethlops). The f i r s t isolate was obtained from a monkey seropositive for HIV, and the others were isolated from monkeys harboring antibodies to the first isolate. These simian retroviruses were referred to as simian immunodeficiency virus from AGM, SIV[AGM], due to their cross-reactivities with HIV structural proteins. These SIV[AGM] isolates were found by Western blotting analysis to have virus-specific proteins of 120,66, 55, 32-40. 24 and 17 kDa, which were all similar in size to the analogous proteins of HIV. Putative gag proteins of p55, p24 and p17 were recognized by sera of human AIDS patients, but the corresponding env proteins of 32-40 and 120 kDa showed only weak cross-reactivity with those of HIV. The transmembrane glycoproteins of these 3 SIV[AGM] isolates showed size heterogeneity, being 32.35 and 40 kDa. This virus had particles that were morphologically similar to those of HIV, and had Mg*+-dependent reverse transcriptase. Furthermore, the SIV[AGM] showed tropism and cytopathic effects on CD4-positive human cell lines. In a sero-epidemiological survey of SIV[AGM] in various non-human primates, 2 other African monkey species, the mandrill and de Brazza's monkey, were also found to have antibodies to SIV[AGM]. These HIV-related simian retroviruses will be important in determining the origin and transmission of HIV group viruses, and may provide useful animal models for studies on the infection and pathogenesis of HIV and AIDS.
Independently, we have tested 1,577 sera from 49 species of non-human primates for the presence of antibodies to HIV, and found one African green monkey (AGM-1) with specific antibodies to HIV (Hayami et al., 1985). We subsequently tried to isolate the simian retrovirus from African green monkeys with antibodies to HIV.
This communication reports the isolation of simian retroviruses similar to HIV (which we named simian immunodefiency virus) from AGM, SIV[AGM], according to the recommendations of the International Committee on the Taxonomy of Viruses (1986), and a seroepidemiological survey of non-human primates for antibodies to SIV [AGM] .
MATERIAL AND METHODS
Recently, simian retroviruses similar to HIV were isolated ' Onray gradient centrifugation* 'TO whom reprint requests should be addressed.