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Isolation of RNA Aptamers for Biological Cofactors by In Vitro Selection

✍ Scribed by Dipl.-Chem. Petra Burgstaller; Dr. Michael Famulok


Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
466 KB
Volume
33
Category
Article
ISSN
0044-8249

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## Abstract __SELEX was used to create an RNA aptamer targeted to protein tyrosine phosphatase 1B (PTP1B), an enzyme implicated in type 2 diabetes, breast cancer and obesity. We found an aptamer that strongly inhibits PTP1B in vitro with a__ K__~i~ of less than 600 pM. This slow‐binding, high‐affin