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Isolation of ras GTP-Binding Mutants Using an in situ Colony-Binding Assay

โœ Scribed by Larry A. Feig, Bin-Tao Pan, Thomas M. Roberts and Geoffrey M. Cooper

Book ID
123632885
Publisher
National Academy of Sciences
Year
1986
Tongue
English
Weight
1010 KB
Volume
83
Category
Article
ISSN
0027-8424

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We have introduced point mutations in v-rap to study their effects on biochemical and biological properties of the ras-encoded protein pZ I. Several of these mutant proteins do not bind GTP and thus lack GTPase activity, while others were shown to have their GTP binding reduced. We have introduced t