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Isolation of polymorphic microsatellite loci from the tapeworm Echinococcus multilocularis

โœ Scribed by Minoru Nakao; Yasuhito Sako; Akira Ito

Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
146 KB
Volume
3
Category
Article
ISSN
1567-1348

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โœฆ Synopsis

Two microsatellites were isolated from a genomic library of Echinococcus multilocularis. The microsatellites, designated EMms1 and EMms2, consist of tandem repeats of CAC-trinucleotide unit. Southern blot hybridization suggests that each of them is a single locus. Using fox-derived wild tapeworms (N=104), PCR-amplification of microsatellites was performed to assess the usefulness of these loci. We found four alleles of EMms1 and two alleles of EMms2. The heterozygosities observed were 10.6% in EMms1 and 7.7% in EMms2. The result suggests that both selfing and outcrossing occur in the adult stage of E. multilocularis.

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EmsB, a tandem repeated multi-loci micro
EmsB, a tandem repeated multi-loci microsatellite, new tool to investigate the genetic diversity of Echinococcus multilocularis
โœ J.M. Bart; J. Knapp; B. Gottstein; F. El-Garch; P. Giraudoux; M.L. Glowatzki; H. ๐Ÿ“‚ Article ๐Ÿ“… 2006 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 534 KB

In order to explore the genetic diversity within Echinococcus multilocularis (E. multilocularis), the cestode responsible for the alveolar echinococcosis (AE) in humans, a microsatellite, composed of (CA) and (GA) repeats and designated EmsB, was isolated and characterized in view of its nature and