Isolation of plasma membrane from xenopus oocyte and it's intramembrane particles
β Scribed by Fujitsuka, Noriaki; Inoue, Masumi; Yoshimura, Atsushi; Fujitsuka, Chie; Sokabe, Masahiro
- Book ID
- 122100348
- Publisher
- Elsevier Science
- Year
- 1991
- Weight
- 85 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0921-8696
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## Abstract We used atomic force microscopy (AFM) to characterize the plasma membrane of __Xenopus laevis__ oocytes. The samples were prepared according to novel protocols, which allowed the investigation of the extraβ and intracellular sides of the membrane, both of which showed sparsely distribut
## Abstract Atomic force microscopy (AFM) was used to investigate the native plasma membrane of __Xenopus laevis__ (__X. laevis__) oocyte purified by means of ultracentrifugation on sucrose gradient and subsequently adsorbed on mica leaves through a physisorption process. Reproducible AFM topograph