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Isolation of Pinus radiata Genomic DNA Suitable for RAPD Analysis

✍ Scribed by Claudia Stange; Doris Prehn; Patricio Arce-Johnson


Book ID
110251304
Publisher
Springer
Year
1998
Tongue
English
Weight
14 KB
Volume
16
Category
Article
ISSN
0735-9640

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Acrylamide gels which arc crosslinked by disultide bridges are found to be an excellent electrophoretic medium for DNA restriction fragments over a wide size range. The gels are solubilised by mercaptoethanol and the fragments are rapidly and conveniently recovered on DEAE~cellulose columns. Fragmen