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Isolation of phosphatidylinositol from soybean phosphatides

✍ Scribed by É. Ya. Kostetskii; N. I. Gerasimenko


Publisher
Springer
Year
1984
Tongue
English
Weight
351 KB
Volume
20
Category
Article
ISSN
0009-3130

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Breakdown of phosphatidylinositol in soy
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We have investigated the breakdown of membrane-bound phosphatidylinositol (PI) in homogenates of soybean (Glycine max) callus. The breakdown of PI was stimulated by the detergent deoxycholate. At pH 7.0 and 1·gl(-1) of deoxycholate the loss of PI was rapid and extensive: more than 80% was broken dow

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## Abstract Type I phosphatidylinositol 4‐phosphate 5‐kinase (PI4P5K) catalyzes the phosphorylation of phosphatidylinositol 4 phosphate [PI(4)P] at carbon 5, producing phosphatidylinositol 4,5 bisphosphate [PI(4,5)P2]. Phosphatidic acid (PA) activates PI4P5K in vitro and plays a central role in the