Isolation of parafluorophenylalanine-resistant mutants from HeLa cell cultures
β Scribed by Lester K. C. Yim; W. Dorsey Stuart
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 454 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0006-2928
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The goal of this work was to begin a genetic study of the molecular mode of action of abscisic acid (ABA), by isolating variant cultured cells resistant to the hormone, or to a factor which induces ABA synthesis, namely water stress. Cell cultures of Nicotiana tabacum L. cv. Wisconsin 38 and N. silv
Variant clones were isolated from Nicotiana silvestris Speg. et Comes cell cultures at low frequencies following severe abscisic-acid (ABA) or mannitol-induced water-stress treatments of plated cells. N. tabacum L. variants were not recovered. Variants from the ABA selection experiments exhibited a