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Isolation of osteosarcoma-associated amplified DNA sequences using representational difference analysis

✍ Scribed by Annet Simons; Irene M. Janssen; Ron F. Suijkerbuijk; René P.H. Veth; Maciej Pruszczynski; Christina A. Hulsbergen-van de Kaa; Stanislas du Manoir; Ad Geurts van Kessel

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
134 KB
Volume
20
Category
Article
ISSN
1045-2257

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✦ Synopsis

Comparative genomic hybridization analysis of a primary osteosarcoma and its metastasis revealed two regions of DNA amplification, one at 17p11.2-12 and one at 19q12-13. Subsequent representational difference analysis of the primary tumor resulted in the isolation of two distinct tumor-amplified DNA fragments originating from chromosome 19. A YAC clone corresponding to one of the two isolated DNA fragments was used for fluorescence in situ hybridization on normal human lymphocyte metaphases and tumor-derived nuclei. This resulted in the localization of this YAC to 19q12-13.1 and confirmed the amplification status of the isolated fragment in the tumors. The availability of such RDA-isolated sequences may be instrumental in the search for genes relevant for tumor development.

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