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Isolation of oligogalacturonic acids up to DP 20 by preparative high-performance anion-exchange chromatography and pulsed amperometric detection

✍ Scribed by Arland T. Hotchkiss Jr; Stephanie L. Lecrinier; Kevin B. Hicks


Book ID
108309277
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
108 KB
Volume
334
Category
Article
ISSN
0008-6215

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Analysis of pectate lyase-generated olig
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Traditionally, UV absorption detection (A,,,) of 4,5-unsaturated bonds has been used to evaluate the depolymerization of pectic polysaccharides by pectate lyase (PLI. This approach ignores the generation of oligogalacturonic acids without a 4,5unsaturated function that appear early in the time cours