✦ LIBER ✦

Isolation of nuclei from melittin-destabilized cells

✍ Scribed by Peter J. Smith; Martin H. Friede; Brenda J. Scott; Claus von Holt

Book ID: 102630226
Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 398 KB
Volume: 169
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90301-6

No coin nor oath required. For personal study only.

✦ Synopsis

Melittin, the hydrophobic polypeptide from bee venom, sufficiently destabilizes the plasma membrane of cultured cells to allow cell disruption in the absence of detergents with minimal homogenization. Nuclei are thus isolated in high yield with intact nuclear membranes and high transcriptional activity.

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