Isolation of murine microglial cells for RNA analysis or flow cytometry
โ Scribed by Cardona, Astrid E; Huang, DeRen; Sasse, Margaret E; Ransohoff, Richard M
- Book ID
- 109945944
- Publisher
- Nature Publishing Group
- Year
- 2006
- Tongue
- English
- Weight
- 264 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1750-2799
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## Abstract ## Background: RNA from sorted cell populations is crucial in many instances. We therefore compared four current protocols for RNA isolation, with regard to mRNA yield and purity. Moreover, we examined the effects on RNA recovery caused by different storage reagents. ## Methods: Smal