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Isolation of monocytes from human peripheral blood using immuno-affinity expanded-bed adsorption

โœ Scribed by L. B. Ujam; R. H. Clemmitt; S. A. Clarke; R. A. Brooks; N. Rushton; H. A. Chase


Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
376 KB
Volume
83
Category
Article
ISSN
0006-3592

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โœ R. H. Clemmitt; H. A. Chase ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 202 KB ๐Ÿ‘ 1 views

The facilitated downstream processing of an intracellular, polyhistidine-tagged protein, glutathione Stransferase [GST-(His) 6 ], direct from unclarified E. coli homogenates using expanded beds of STREAMLINE chelating, has been investigated. A series of pilot experiments were used to develop prepara