✦ LIBER ✦

Isolation of metastatic B16 melanoma variants using anti-Met 72 monoclonal antibodies and flow cytometry

✍ Scribed by Jianhua Xiang; Arthur K. Kimura

Publisher: Springer
Year: 1987
Tongue: English
Weight: 457 KB
Volume: 5
Category: Article
ISSN: 0262-0898
DOI: 10.1007/bf00116624

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✦ Synopsis

Analysis of a number of B16 melanoma clones has revealed a high correlation between metastatic activity and the quantitative expression of a 72 000 dalton glycoprotein, Met 72. In the present study, metastatic tumor cell variants have been directly isolated from a heterogeneous, poorly metastatic melanoma (B16-F1) by anti-Met 72 monclonal antibodies and cell sorting procedures. These studies provide direct proof that Met-72 antigens are in fact surface markers of B16 melanoma metastatic variants and may provide the means of monitoring their presence, influence and autonomy during tumor progression.

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✍ Nanette P. Parratto; Arthur K. Kimura 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 787 KB

Metastatic variants of the B 16 melanoma displaying high experimental metastatic potential have been shown to express high levels of a 72,000-dalton glycoprotein (Met-72) on their cell surface (Kimura AK, Xiang J: J Nut Can Znst 76: [1247][1248][1249][1250][1251][1252][1253] 1986). Monoclonal antibo