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Isolation of mammalian transfer RNA

โœ Scribed by H. Rogg; W. Wehrli; M. Staehelin


Book ID
115743672
Publisher
Elsevier Science
Year
1969
Weight
181 KB
Volume
195
Category
Article
ISSN
0005-2787

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Rapid isolation of total RNA from mammal
โœ Mark Emmett; Barbara Petrack ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 1007 KB

A rapid procedure for the isolation of total RNA from small amounts of mammalian tissue (35 to 150 mg) is described. Tissues were homogenized in the presence of RNase inhibitors but in the absence of strong detergents. Contaminants were removed by phenol/chloroform extraction and Sephadex column chr