Isolation of lysozyme on chitosan
β Scribed by Riccardo A. A. Muzzarelli; Gaetano Barontini; Roberto Rochetti
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 317 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0006-3592
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The conditions for preparing partially IV-acetylated chitosans (PNACs) by reaction of chitosan with acetic anhydride in homogeneous solution were investigated. The following conditions are recommended: chitosan, 1%; acetic acid, 0.4%; methanol, 50%; at room temperature. About 80% of the acetic anhyd
## Abstract Frog Lysozyme has been purified by sequential application of acid extraction, salt fractionation, CMβcellulose chromatography, heat treatment, and gel filtration. Eight isozymes of purified lysozyme were found to be stable during prolonged storage. Isozymes were separated by preparative
tories. Its chemical and textural characteristics have been Hen egg white lysozyme has been succinylated, the isoelectric reported before under the name HAP III (2); its surface area point being shifted thereby from about 11 to 4.5-4.7. Its adsorption is 80 m 2 rg 01 . on a chromatography grade hyd