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Isolation of leukocytes infiltrating the islets of Langerhans of diabetes-prone mice for flow cytometric analysis

✍ Scribed by Christelle Faveeuw; Marie-Claude Gagnerault; Françoise Lepault


Book ID
113181782
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
743 KB
Volume
187
Category
Article
ISSN
0022-1759

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Adult CBA/HZgb mice islets harvested by collagenase digestion were injected intraperitoneally in 52 singeneic diabetic recipients (CBA/Hgb leads to CBA/HZgb, 450-600 islets per mouse). Normal serum glucose levels, 24-h urine volume, insulin levels and body weight were completely restored to normal i