Isolation of L-ethionine resistant cell lines inVigna sinensisL. after mutagen treatment

✍ Scribed by Rina Ahmed; S. Dutta Gupta; P. D. Ghosh

• Book ID: 104620367
• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 450 KB
• Volume: 7
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00043037

✦ Synopsis

Chemical mutagenesis was employed for the isolation of variant cell lines resistant to L-Ethionine (L-Eth) in Vigna sinensis L. We have measured cell survival after treatment of Vigna sinensis cell suspensions with different mutagens: Ethyl methanesulfonate (EMS), N-methyl-N-nitrosoguanidine (NTG) and Acridine Orange (AO). NTG was more toxic than EMS and AO.

L-Eth resistant colonies were isolated by plating on selective medium after NTG treatment. The frequencies of appearance of resistant cells of MS-3 media supplemented with 20 t~g/ml L-Eth were 1.3 × 10 -6 to 1.8 × 10 -5. The highest number of L-Eth resistant caUi were recorded in cells treated with 10t~g/ml NTG. Few resistant colonies also appeared spontaneously from non-mutagenized cultures with a frequency of 2.9 × 10 -7 to 5.7 × 10 -7. However, a number of isolated colonies were discarded after successive retesting. The number of resistant calli dropped from 204 to 22 during successive retests. The significance of these observations has also been discussed.