Isolation of induced mutations in E. coli affecting the electrophoretic mobility of enzymes
β Scribed by Charles R. Shaw; James N. Baptist; David A. Wright; Thomas S. Matney
- Book ID
- 116036702
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 842 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0027-5107
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Amber mutants (am3 and am4) of Escherichia coli K12 defective in the synthesis of nusA protein (Friedman 1971) were isolated from a strain harboring an amber suppressor (sup-126) that is active only at low temperatures. These mutants grew at low temperature (30 degrees C) but did not grow at tempera
A method to obtain amber mutations in ribosomal protein genes is described. tit relies on the P1-mediated localized mutagenesis (Hong and Ames, 1971) and on the fact that the recipient strain contains (a) an efficient but genetically unstable suppressor, (b) a particular thermoinducible lambda proph