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Isolation of human liver angiotensin-converting enzyme by chromatofocusing

✍ Scribed by Ivan Yu. Sakharov; Sergei M. Danilov; Nadejda V. Sukhova

Book ID
107714502
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
268 KB
Volume
166
Category
Article
ISSN
0003-2697

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A relatively simple procedure is described for purifying human serum angiotensin-converting enzyme. The enzyme was purified 130,000-fold to electrophoretic homogeneity using affinity chromatography as the principal purification step. The ligand was an immobilized competitive inhibitor, D-cysteinyl-L