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Isolation of human erythrocyte membranes in glucose solution

✍ Scribed by Akio Tomoda; Kenichi Kodaira; Akira Taketo; Kazuo Tanimoto; Yoshimasa Yoneyama

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
710 KB
Volume
140
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described for the preparation or removal of erythrocyte membranes from hemolysates by a glucose solution. The procedure is simple and rapid, requiring centrifugation at 8000g for 2 min. The preparation has microscopic shape and two-dimensional peptide patterns similar to those of the membrane isolated by conventional procedures (10,000g for 20 min). The present procedure is suitable for dealing with a bulky preparation or for removal of erythrocyte membranes from large volumes of hemolysates to purify enzymes and proteins of soluble or membrane fractions.

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