Isolation of highly purified, functional carboxy-terminally truncated hepatitis B virus middle surface protein activators from eucaryotic expression systems
✍ Scribed by E Hildt; S Urban; C Eckerskorn; P H Hofschneider
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 365 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
genes for the proliferation of hepatocytes might be transacti-Carboxy-terminally truncated hepatitis B virus vated by each of these activators. (HBV) middle surface proteins (MHBs t ) show a tran-Coding sequences for the carboxy-terminally truncated scriptional activator function. Two different subtypes of hepatitis B virus middle surface protein (MHBs t ) transcrip-MHBs t activators can be distinguished: an ER-localized tional activator were first identified in the surface gene (pretype, represented here by MHBs t76 (truncated at amino S/S) of integrated HBV DNA subcloned from the HBV-associ-Abbreviations: HBV, hepatitis B virus; HCC, hepatocellular carcinoma; MHBs t , carboxy-terminally truncated hepatitis B virus middle surface protein; Sf9, Spodoptera frugi-tional modifications were investigated by mass spectrometry. perda; AcNPV, Autographa Californica nuclear polyhedrosis virus; CAT, chloramphenicol-The functionality of these proteins was demonstrated by elecacetyl-transferase; SDS, sodium dodecyl sulfate. tric-field-mediated transfer into reporter cells.