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Isolation of high-specific-activity subunits of cholera toxin by reversed-phase high-performance liquid chromatography

✍ Scribed by Stephen D. Pearson; James D. Dixon; Steven F. Northwehr; Alexander Kurosky

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
682 KB
Volume
359
Category
Article
ISSN
1873-3778

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Baseline separation of subunits of diverse phycobiliproteins was achieved by a reverse-phase HPLC gradient method with a C4 large-pore column and a solvent system consisting of 0.1% trifluoroacetic acid (TFA) in water and 0.1% TFA in 2:1 (v/v) acetonitrile:isopropanol. The procedure was successfully