Isolation of enamelinlike proteins from blue shark (Prionace glauca) enameloid

✍ Scribed by Graham, Edward Earl

• Book ID: 102892578
• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 542 KB
• Volume: 234
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402340203

✦ Synopsis

Abstract

A sequential dissociative extraction scheme was used to extract proteins from developing Blue Shark enameloid. The first extraction solution (4 M guanidine HCl) solubilized the polypeptides, mainly collagenous, not closely associated with the hydroxyapatite. The next extraction solution (4 M guanidine HCl, 0.5 M ethylenediaminetetraacedic acid (EDTA)) solubilized the proteins more closely associated with the tooth mineral component. After extraction, the proteins were separated and isolated with gel electrophoresis. Protein molecular weights were determined and selected proteins were isolated for amino acid composition analysis. The two proteins isolated were tested for mammalian enamel protein antigenic determinants by a “Dot” immunobinding assay. The isolated proteins were enamelinlike by extraction criteria and amino acid composition. Further, the two proteins share antigenic determinants with mammalian enamel proteins.