𝔖 Bobbio Scriptorium
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Isolation of DNA from blood

✍ Scribed by Teresa L. Kendall; Darryl J. Byerley; Roger Dean

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
862 KB
Volume
195
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid, economical method of DNA isolation from blood was developed that yields DNA suitable for Southern analysis and polymerase chain reactions without organic solvent extractions. Bovine DNA was prepared from peripheral leukocytes and nuclei using pronase E digestion and ethanol precipitation. This isolation method readily adapts to multiple samples. The DNA is characterized by high yield, solubility, lack of protein contamination, and ease of restriction endonuclease digestion.

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Endonuclease analyses of DNA of human he
Endonuclease analyses of DNA of human herpesvirus-6 isolated from blood before and after bone marrow transplantation
✍ Tetsushi Yoshikawa; Toshihiko Nakashima; Yoshizo Asano; Sadao Suga; Takehiko Yaz πŸ“‚ Article πŸ“… 1992 πŸ› John Wiley and Sons 🌐 English βš– 462 KB

## Abstract Three strains of human herpesvirus‐6 (HHV‐6) were isolated from peripheral blood mononuclear cells of a leukemic child with the antibody of HHV‐6 before and after bone marrow transplantation (BMT); two strains were obtained before BMT and one after BMT. The DNA extracted from the three