ISOLATION OF CYTOCHROME OXIDASE COMPLEX FROM SEA URCHIN MITOCHONDRIAL INNER MEMBRANES
β Scribed by TEOFANI KUTRA; MARINA TIMONERI; ANTONINO OLIVA; ANNA MARIA RINALDI
- Book ID
- 102565698
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 254 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1065-6995
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β¦ Synopsis
Cytochrome oxidase complex has been isolated and purified from sea urchin mitochondrial inner membranes. The complex exhibited a discrete number of bands in PAGE and a 17 kDa band was recognized by an anti-human subunit IV antibody. No bands were recognized by an anti-yeast hsp60 antibody and an anti-sea urchin ATPase subunit.
π SIMILAR VOLUMES
Lysis of mitochondria from sea urchin embryos with Triton X-100 led to a complete conversion of DNA-containing mitochondrial residues into protein-DNA complex with a density higher than 1.22 g/cm3 in sucrose solutions. This complex banded isopycnically in metrizamide gradients at a density of 1.26 g