Isolation of cross-reacting compounds to incstar cyclo-trac sp ria in blood samples obtained from cardiac allograft patients on cyclosporine therapy
✍ Scribed by Jack C. K. Loo; Dr. Keith D. Gallicano; Iain J. McGilveray; Nicole Beaudoin; Shiv L. Jindal
- Book ID
- 102307414
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 220 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
✦ Synopsis
To examine the specificity of the lncstar Cyclo-Trac SP RIA kit, individual blood samples from 28 cardiac allograft patients on cyclosporine A (CsA) therapy were extracted and chromatographed by HPLC. Initially, eluates from a pool of the above samples were collected at regular intervals and measured by RIA to locate possible cross-reacting compounds. Unknown cross-reacting materials
were detected in a fraction (UNK) that was collected before elution of CsA. For each patient's sample, fraction UNK and the fraction containing CsA were then collected and measured by RIA. In 9 of 28 samples, crossreactivity was detected in fraction UNK, range 11 to36%, mean 22 k 7 5% Cross-reactivity was not apparent in fraction UNKof CsA-free blood samples from normal volunteers