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Isolation of Circular DNA Molecules from Whole Cellular DNA by Use of ATP-Dependent Deoxyribonuclease

✍ Scribed by Tsunehiro Mukai, Kenichi Matsubara and Yasuyuki Takagi


Book ID
123652077
Publisher
National Academy of Sciences
Year
1973
Tongue
English
Weight
605 KB
Volume
70
Category
Article
ISSN
0027-8424

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Kinetics of circular DNA molecule digest
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A model for kinetics of circular substrate cleavage by restriction endonuclease was formulated. The aim of the analysis of the model was to extract kinetic constants for all target sites from time-dependence of fragment concentration in reaction products. That was proved to be possible for molecules