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Isolation of cDNAs from maternal mRNAs specifically present during early development

โœ Scribed by Jacques Moreau; Nathalie Iouzalen; Marcel Mechali


Book ID
102951643
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
728 KB
Volume
41
Category
Article
ISSN
1040-452X

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โœฆ Synopsis


In many animals the regulation of early embryonic development is under the control of mRNAs of maternal origin. We describe here the construction and characterization of a subtractive cDNA library to isolate genes whose expression is restricted to early development. Such genes might be potentially involved in developmental diseases, if accidentally expressed during adult life. Seven yet unknown genes preferentially expressed in the oocyte and present during early development were isolated from this library by differential screening. Their transcription patterns provide evidence for a selective expression during oogenesis and the existence of a gene family specifically implicated in functions linked to early embryogenesis. o 1995 WiIey-Liss, Inc.


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