Isolation of CD34-Positive Hematopoietic Progenitor Cells
β Scribed by Elizabeth J. Shpall; Ursula Gehling; Pablo Cagnoni; Malcolm Purdy; Lisa Hami; Adrian P. Gee
- Book ID
- 118768964
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 657 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1058-6687
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High-dose chemotherapy with autologous hematopoietic progenitor-cell support is increasingly used for the treatment of hematologic malignancies and solid tumors. Over the last few years, the major source of progenitor cells for clinical use has shifted from bone marrow to peripheral blood. The curre
In an effort to obtain defined culture conditions for ex vivo expansion of hematopoietic stem and progenitor cells which avoid the supplementation of serum, we cultured human CD34 + hematopoietic progenitor cells in a chemically defined, serum-free medium in the presence of hematopoietic growth fact