Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida
✍ Scribed by Dr. Pierre E. Rollin; Thomas G. Ksiazek; Luanne H. Elliott; Eugene V. Ravkov; Mary Lane Martin; Sergey Morzunov; Walter Livingstone; Marty Monroe; Gregory Glass; Sup Ruo; Ali S. Khan; James E. Childs; Stuart T. Nichol; C. J. Peters
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 532 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
Numerous rodents were trapped for serologic and virologic studies following the identification of a hantavirus pulmonary syndrome (HPS) case in Dade County, Florida. Cotton rats (Sigrnodun hispidus) were the most frequently captured rodent and displayed the highest seroprevalence to a variety of hantavirus antigens. Hantavirus genome RNA was detected in all the seropositive cotton rats tested, using a reverse transcriptasepolymerase chain reaction (RT-PCR) assay. A virus was isolated from tissues of two seropositive cotton rats by cultivation of lung and spleen homogenates on Vero E6 cells. Nucleotide sequence information obtained by direct RT-PCR and the serologic relationships of this virus with the other hantaviruses indicate that this virus, Black Creek Canal virus, represents a new hantavirus distinct from the previously known serotypes.