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Isolation of auxotrophic mutants and development of conjugation system in KISR methylotrophs

✍ Scribed by M. Kadri; F. Al-Salameen; S. Haditirto; J. Engel

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
351 KB
Volume
10
Category
Article
ISSN
0138-4988

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✦ Synopsis

Isolation of auxotrophic mutants and conjugal transfer of plasmids are successfully demonstrated in four obligate methylotrophic strains isolated a t Kuwait Institute for Scientific Research (KISR). Out of 11 auxotrophic, mutants, three required methionine and two leucine. Complete transfer of plasmid R68.45 from Pseudomoms to KISR methylotrophs and vice versa have been achieved with a low transfer rate.

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