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Isolation of an Organ-Specific Leucyl-tRNA Synthetase from Soybean Seedling

โœ Scribed by Jan Kanabus and Joe H. Cherry


Book ID
123650159
Publisher
National Academy of Sciences
Year
1971
Tongue
English
Weight
746 KB
Volume
68
Category
Article
ISSN
0027-8424

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A procedure for the large-scale isolation of leucyl-tRNA synthetase from E. cole MRE 600 is described: The enzyme was purified about 320-fold to homogeneity by precipitation with cetyl-trimethyl-ammonium bromide, two consecutive chromatographies on DEAE-cellulose and three on hydroxyapatite with an